For me it's:
Demon's Souls: Old King Allant
Dark Souls: Knight Artorias
Dark Souls 2: Sir Alonne (my favorite boss in all of Soulsbourne)
Bloodborne: Lady Maria
Dark Souls 3: The Nameless King
I don't consider Sekiro a Soulsborne game.
Dark Souls 2: Sir Alonne
Bloodborne: Ludwig, the Accursed/the Holy Blade
Dark Souls 3: Slave Knight Gael
Sekiro: Owl (Father)
DeS - Maiden Astrea
Ds1 - Artorias
Ds2 - Alonne
BB - Ludwig
Ds3 - Gael
Elden Ring - Glaive Master Hodir
And the award for the most generic answers goes to...
DES. Dragon god.
DS1. Cannot decide between the DLC bosses because all of them are so great!
DS2. Fume knight.
DS3. Midir.
Bloodborne. Lady Maria.
What do you think?